Update from the lab

Yesterday I gave a presentation to the lab on my work from the past several weeks, which can be found here. Last week we received the famous Alexa Fluor dyes, which I will use to label DnaK at two different positions for FRET experiments. So far I've studied the fluorescent properties of the dyes on three different fluorimeters and run some labeling reactions to determine how fast the dyes are conjugated onto DnaK. Determining the reaction rates for different DnaK mutants will be crucial for selectively labeling the protein at distinct residues. The mutant I tested this week, T136C/C15S, reacted in seconds, so hopefully another mutant will react slower.